When counting, employ a system whereby cells are only counted when they are set within a square or on the right-hand or bottom boundary line. Using a hand tally counter, count the cells in one set of 16 squares.Focus on the grid lines of the hemocytometer with a 10X objective.Thus the volume of the counted culture can be calculated and with it the concentration of the cells.įigure 1: Manual hemocytometer, showing in detail the grid to count cells. Looking at the sample under the microscope, the researcher uses the grid to manually count the number of cells in a certain area. A drop of a cell culture is placed in the sink. ![]() ![]() The slide has a sink in its middle the area of the sink is marked with a grid. A hemocytometer is a microscope slide that is specially designed to enable cell counting. To count the cells manually you need to use a hemocytometer.
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